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Movies Prepared by Students in the Course:
Dogterom Movie 1: force generating and buckling microtubules in round mutant cells (mor2-phenotype) that lacked the microtubule bundling protein ase1p.
EB1-GFP Movie: Drosophila S2 cells are expressing EB1-GFP, a protein that localizes selectively to the growing ends of microtubules (punctate fluorescence shown in the movie reflects these microtubules growing towards the periphery). Imaging with a Nikon microscope with a Solamere spinning disk confocal attachment and Hamamatsu Orca camera.
T cell movie: A Jurkat T cell is adhering to a biplanar lipid bilayer coated containing anti-TCR antibodies (red). A stable contact of T cell with the bilayer is shown by the formation of a central signaling zone (called the c-SMAC; red). Superimposed in green is a movie of single molecules ICAM-1 incorporated in the planar bilayer. These molecules are diffusing rapidly in the artificial bilayer but occasionally become trapped at the periphery of the c-SMAC. See section on Vale research projects. Imaging was performed with a Zeiss Axiovert with their laser illumination total internal reflection module and a recorded with a Stanford Photonics intensified camera.
Tracking kinetochore fibers using a MatLab-based algorithm:
Left, a movie of tubulin-GFP in mitosis in S2 cells shows thick bundles of microtubules attached to chromosomes (kinetochore fibers). On the right, a student in the course wrote a Matlab code that tracks the postions of these kinetochore fibers. See Vale research section. |
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